Protocol for Disome Profiling to Survey Ribosome Collision in Humans and Zebrafish

Protocol for Disome Profiling to Survey Ribosome Collision in Humans and Zebrafish

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Summary: Ribosomes often encounter obstacles during translation elongation and thus collide with each other.Disome profiling, an optimized ribosome profiling method, specifically sequences the long ribosome footprints generated from collided ribosomes produced by the ribosome pause and thus allows the Nutritionals - Standard Tube Feeding survey of sites in a genome-wide manner.This protocol details the procedure from lysate preparation of human tissue Armchair cultures and zebrafish embryos to sequencing library construction.

For complete details on the use and execution of this protocol, please refer to Han et al.(2020).